A new publication by the Analytical Chemistry group of Prof. Rainer Bischoff, University of Groningen, one of the members of the BiomarkerBay network.
The publication reports a simplified immunoprecipitation in 96-well ELISA format (IPE) approach for fast and easy enrichment of proteins. The applicability of this approach for enriching low-abundant proteins was demonstrated by an IPE-based quantitative workflow using liquid chromatography-mass spectrometry (LC-MS) for the soluble Receptor of Advanced Glycation End-products (sRAGE), a promising biomarker in chronic obstructive pulmonary disease (COPD).
The method was validated according to U.S. Food and Drug Administration (FDA) and European Medicines Agency (EMA) guidelines and enabled accurate quantitation of sRAGE between 0.1 and 10 ng/mL in 50 µL serum.
The assay, which was developed in the group of Prof. Rainer Bischoff, Analytical Chemistry, is described in:"A fully validated liquid chromatography-mass spectrometry method for the quantification of the soluble receptor of advanced glycation end-products (sRAGE) in serum using immunopurification in a 96-well plate format" (Klont et al., Talanta. 2018 May 15;182:414-421). Here is the full publication.