A new publication by the Analytical Chemistry group of Prof. Rainer Bischoff, University of Groningen, one of the members of the BiomarkerBay network.
The publication presents an affimer-based liquid chromatography-mass spectrometry (LC-MS) method for quantification of the soluble Receptor of Advanced Glycation End-products (sRAGE), a promising biomarker for chronic obstructive pulmonary disease (COPD). The method was validated according to European Medicines Agency and U.S. Food and Drug Administration guidelines and enabled quantitation of serum sRAGE between 0.2 and 10 ng/mL. Comparison between the affimer-based method and a previously developed, validated antibody-based method showed good correlation (R2 = 0.88), and indicated that 25% lower sRAGE levels are reported by the affimer-based assay.
This is the first-time application of affimers in a quantitative LC-MS method, which supports the potential of affimers as robust alternatives to antibodies.
The assay, which was developed in the group of Prof. Rainer Bischoff, Analytical Chemistry, is described in:"Affimers as an Alternative to Antibodies in an Affinity LC-MS Assay for Quantification of the Soluble Receptor of Advanced Glycation End-Products (sRAGE) in Human Serum." (Klont et al., J Proteome Res. 2018 Jul 13). Here is the full publication.
